Bioautography to assess antibacterial activity of Ottonia martiana Miq. (Piperaceae) on the human oral microbiota

Miriam  Machado Cunico; Celso Garcia Auer; Cristina Peitz de Lima; Lilian Cristina Côcco; Carlos Itsuo Yamamoto; Marilis Dallarmi Miguel; Obdulio  Gomes Miguel; Carlos Roberto  Sanquetta

Miriam Machado Cunico
Celso Garcia Auer
Cristina Peitz de Lima
Lilian Cristina Côcco
Carlos Itsuo Yamamoto
Marilis Dallarmi Miguel
Obdulio Gomes Miguel
Carlos Roberto Sanquetta

Keywords: Amides. Antibacterial activity. Bioautography. Toothache. Ottonia martiana.

Abstract

Ottonia martiana Miq. (Piperaceae), a plant known popularly in southern Brazil as “anestésia” and used in the treatment of odontalgia for its anesthetic action on the oral mucosa, was investigated for antibacterial activity by paper disc agar diffusion and bioautographic methods, against microorganisms present in the human oral cavity [Streptococcus mutans (ATCC 25175), Streptococcus mitis (ATCC 49456), Streptococcus pyogenes (ATCC 19615), Streptococcus salivarius (ATCC 25975), Escherichia coli (ATCC 11229 and 25922), Pseudomonas aeruginosa (ATCC 27853) and Enterobacter aerogenes(ATCC 27853).The crude extract of O. martiana (32.9 mg mL-1) had antibacterial potential against all Gram-positive bacteria tested. Analysis of the bioautograms led to the detection of bioactive substances, among which it was possible to identify piperovatine (Rf 0.35), piperlonguminine (Rf 0.52) and isopiperlonguminine (Rf 0.52). The piperovatine and isopiperlonguminine were isolated from the roots of O. martiana, guided by a bioautographic antibacterial bioassay.